The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one

Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). Female eels need to ovulate within 18 h after release good quality eggs. Progesterone (P), an upstream precursor DHP, may promote endogenous DHP production and improve egg quality. The purpose this study was therefore compare treatment P with on batch level, vitro , determine dose-response effects, vivo at a single dose. For experiment, ovarian tissue extracted placed culture plates containing hormone-free medium media supplemented treatment: 1, 10 100 ng mL −1 or 10, 1,000 . At start incubation, folliculated oocytes were sampled for histology, microscopy qPCR. After incubation 12 h, qPCR analysis. females either injected dose 2 mg kg assess their effects ovulation reproductive success. moment release, eggs RNA sequencing expression genes involved aspects. Remaining fertilized larval viability recorded. Both able induce GVBD (DHP ) Expression oocyte maturation similar both treatments. Regarding results, RNAseq results reflected Females ovulated, released eggs, equally produce larvae without any differences Our support conclusion that work well more attractive apply price 3,000 times lower than DHP.